Reactome | ERCC1:XPF cleaves flaps generated by SSA

ERCC1:XPF cleaves flaps generated by SSA

Stable Identifier

R-HSA-5686657

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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ERCC1:XPF cleaves flaps generated by SSA

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The endonuclease complex ERCC1:XPF (ERCC1:ERCC4) is recruited to single strand annealing (SSA) sites of DNA double-strand break (DSB) repair through direct interaction between XPF (ERCC4) and RAD52 (Motycka et al. 2004). ERCC1:XPF cleaves the ssDNA flaps generated by displacement of non-complementary 3' parts of 3' ssDNA overhangs during RAD52-mediated annealing. The enzymatic activity of ERCC1:XPF is necessary for the completion of SSA (Motycka et al. 2004, Al-Minawi et al. 2008, Ahmad et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
18541667	ERCC1-XPF endonuclease facilitates DNA double-strand break repair van Drunen, E, Ahmad, A, Beverloo, HB, Niedernhofer, LJ, Robinson, AR, Weisberg, DB, Hasty, P, Duensing, A, Hoeijmakers, JH	Mol. Cell. Biol.	2008
17962301	The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells Saleh-Gohari, N, Helleday, T, Al-Minawi, AZ	Nucleic Acids Res.	2008
14734547	Physical and functional interaction between the XPF/ERCC1 endonuclease and hRad52 Bessho, T, Post, SM, Motycka, TA, Sung, P, Tomkinson, AE	J. Biol. Chem.	2004