(d)NTP + ADP <=> (d)NDP + ATP (NME1,2,3)

Stable Identifier
R-HSA-482621
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Cytosolic nucleoside diphosphate kinases catalyze the reversible reaction of ribonucleoside and deoxyribonucleoside 5'-diphosphates with ADP to form the corresponding nucleoside 5'-diphosphates and ATP. These kinases are ubiquitously expressed enzymes with broad substrate specificities (Berg and Joklik 1954; Parks and Agarwal 1973). Three human cytosolic nucleoside diphosphate kinase proteins, NME1, 2, and 3, have been characterized biochemically (Gilles et al. 1991; Schaertl et al. 1998; Erent et al. 2001; Chen et al. 2003). All are catalytically active as hexamers: homohexamers of NME1, 2, and 3 have been described, as have heterohexamers containing all possible combinations of NME1 and 2 (Gilles et al. 1991; Erent et al. 2001).

While the high ratio of ATP to ADP concentrations in the cytosol normally favors the conversion of (d)NDP and ATP to (d)NTP and ADP, the reversibility of the reactions and the overlapping substrate specificities of the enzymes suggest that this group of reverse reactions can buffer the intracellular nucleotide pool and regulate the relative concentrations of individual nucleoside di- and tri-phosphates in the pool.

Literature References
PubMed ID Title Journal Year
12972261 Nucleotide binding to nucleoside diphosphate kinases: X-ray structure of human NDPK-A in complex with ADP and comparison to protein kinases

Chen, Y, Gallois-Montbrun, S, Schneider, B, Veron, M, Moréra, S, Deville-Bonne, D, Janin, J

J Mol Biol 2003
  The Enzymes, 3rd ed

Boyer, PD

  1973
1851158 Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme

Gilles, AM, Presecan, E, Vonica, A, Lascu, I

J Biol Chem 1991
13211603 Enzymatic phosphorylation of nucleoside diphosphates

Berg, P, Joklik, WK

J Biol Chem 1954
9488696 Substrate specificity of human nucleoside-diphosphate kinase revealed by transient kinetic analysis

Schaertl, S, Konrad, M, Geeves, MA

J Biol Chem 1998
11277919 Structural and catalytic properties and homology modelling of the human nucleoside diphosphate kinase C, product of the DRnm23 gene

Erent, M, Gonin, P, Cherfils, J, Tissier, P, Raschella, G, Giartosio, A, Agou, F, Sarger, C, Lacombe, ML, Konrad, M, Lascu, I

Eur J Biochem 2001
Participants
Participant Of
Event Information
Catalyst Activity
Catalyst Activity
Title
nucleoside diphosphate kinase activity of NME1,2 hexamers [cytosol]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created