Reactome | ESPL1 (Separase) cleaves centromeric cohesin

ESPL1 (Separase) cleaves centromeric cohesin

Stable Identifier

R-HSA-2467809

Type

Reaction [transition]

Species

Homo sapiens

Compartment

chromosome, centromeric region, cytosol

ReviewStatus

5/5

Locations in the PathwayBrowser

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Cell Cycle (Homo sapiens)

- Cell Cycle, Mitotic (Homo sapiens)
  - M Phase (Homo sapiens)
    - Mitotic Metaphase and Anaphase (Homo sapiens)
      - Mitotic Anaphase (Homo sapiens)
        
        Separation of Sister Chromatids (Homo sapiens)
        
        ESPL1 (Separase) cleaves centromeric cohesin (Homo sapiens)

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ESPL1 (Separase) cleaves centromeric cohesin

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ESPL1 (separin i.e. separase) cleaves RAD21 (SCC1) subunit of centromeric cohesin at two sites that conform to the consensus separase recognition site E-X-X-R: after arginine residue R172 and after arginine residue R450 (Hauf et al. 2001). Phosphorylation of RAD21 at the serine residue S454 by PLK1 in prometaphase facilitates ESPL1-mediated cleavage of RAD21 at the C-terminal cleavage site R450 (Hauf et al. 2005). The N-terminal and C-terminal RAD21 cleavage fragments remain bound to the rest of the cohesin complex (Deardorff et al. 2012). It is not clear whether RAD21 middle fragment also continues to be associated with cohesin.

Literature References

PubMed ID	Title	Journal	Year
11509732	Cohesin cleavage by separase required for anaphase and cytokinesis in human cells Peters, JM, Hauf, S, Waizenegger, IC	Science	2001
22885700	HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle Shirahige, K, Kiyono, T, Magnaghi-Jaulin, L, Clark, D, Katou, Y, Krantz, ID, Kaiser, FJ, Takagaki, K, Ernst, S, Nozaki, N, Siu, VM, Prigent, C, Christianson, DW, De Baere, E, Decroos, C, Francey, LJ, Di Donato, N, Bando, M, Saitoh, K, Kaur, M, Hirashima, K, Itoh, T, Suzuki, Y, Nakato, R, Mortier, GR, Seimiya, H, Wilde, JJ, Gillessen-Kaesbach, G, Jackson, LG, Watrin, E, Gyftodimou, Y, Willems, PJ, Lombardi, PM, Petersen, MB, Deardorff, MA, Ishikawa, Y, Minamino, M, Jaulin, C, Cole, KE, Hirota, T, Komata, M, Hullings, M	Nature	2012
15737063	Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2 Roitinger, E, Mechtler, K, Dittrich, CM, Peters, JM, Hauf, S, Koch, B	PLoS Biol	2005