Reactome | Viral Polymerase Assembly

Viral Polymerase Assembly

Stable Identifier

R-HSA-192830

Type

Reaction [binding]

Species

Homo sapiens

Related Species

Influenza A virus

Compartment

nucleoplasm

ReviewStatus

5/5

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Disease (Homo sapiens)

- Infectious disease (Homo sapiens)
  - Viral Infection Pathways (Homo sapiens)
    - Influenza Infection (Homo sapiens)
      - Influenza Viral RNA Transcription and Replication (Homo sapiens)
        
        vRNP Assembly (Homo sapiens)
        
        Viral Polymerase Assembly (Homo sapiens)

General

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The mature ternary influenza viral polymerase complex consists of PB1, PB2, and PA. The N-terminus of PB1 (residues 1-48) interacts with PB2, and amino acids 506-659 in PB1 interact with the PA subunit (Gonzalez, 1996; Perez, 2001). Although monomeric PB1, PB2 and PA, as well as PB1-PB2 and PB1-PA dimers are likely to exist in infected cells, it is believed that most of the polymerase proteins are assembled into the trimeric PB1-PB2-PA complex (Detjen, 1987). Newly synthesized subunits of the polymerase are imported into the nucleus through nuclear localization signals (NLS), which interact with cellular importin family proteins (Jones, 1986; Buolo, 2006). Importin beta-3 (Ran binding protein 5) facilitates nuclear import of PB1 and a PB1-PA dimer (Deng, 2006); coexpression of PA with PB1 was shown to enhance the import of PB1 (Fodor, 2004). A PB1-PB2 dimer has been found to interact with the molecular chaperone heat shock protein 90 (HSP90) to facilitate import (Naito, 2007). The three subunits assembled in the nucleus form a mature ternary polymerase complex that binds viral vRNA or cRNA (Jones, 1986; Buolo, 2006).

Literature References

PubMed ID	Title	Journal	Year
3783823	The three influenza virus polymerase (P) proteins not associated with viral nucleocapsids in the infected cell are in the form of a complex Detjen, BM, Katze, MG, St, Angelo C, Krug, RM	J Virol	1987
3023071	Nuclear location of all three influenza polymerase proteins and a nuclear signal in polymerase PB2 Philpott, KL, Reay, PA, Jones, IM	EMBO J	1986
11483758	Functional analysis of PA binding by influenza a virus PB1: effects on polymerase activity and viral infectivity Donis, RO, Perez, DR	J Virol	2001
8948635	Identification of two separate domains in the influenza virus PB1 protein involved in the interaction with the PB2 and PA subunits: a model for the viral RNA polymerase structure Zurcher, T, Gonzalez, S, Ortin, J	Nucleic Acids Res	1996

Participants

Input

Output

Viral Polymerase [nucleoplasm] (Influenza A virus)

Participates

as an event of

vRNP Assembly (Homo sapiens)

This event is regulated

Positively by

Regulator

HSP90AA1 [cytosol] (Homo sapiens)

Regulator

IPO5 [nucleoplasm] (Homo sapiens)

Disease

Name	Identifier	Synonyms
influenza	DOID:8469	Influenza with other manifestations (disorder), Influenza with other manifestations NOS (disorder), flu, Influenza with other manifestations, Influenza with non-respiratory manifestation (disorder), influenza with non-respiratory manifestation

Authored

Garcia-Sastre, A (2007-02-13)

Bortz, E (2007-02-13)

Reviewed

Squires, B (2007-02-13)

Created

Gillespie, ME (2007-02-07)